• Humoral immune responses (ELISA, Virus Neutralization, Plaque Reduction, Haemagglutination Inhibition, etc. )
  • Cell-mediated immune responses;
  • Prime-boost strategies;
  • Production of hyper immune serum;
  • Longevity of protection;
  • Dose-finding, Dose-optimization;
  • Clinical scoring (signs of disease, body temperature, lung function, etc), viral loads (by quantitative PCR and virus titration), gross pathology, histopathology, immune histochemistry, etc.

PLoS One. 2014 May 21;9(5):e93761.

Intranasal H5N1 Vaccines, Adjuvanted with Chitosan Derivatives, Protect Ferrets against Highly Pathogenic Influenza Intranasal and Intratracheal Challenge.

Mann, Noulin, Catchpole, Stittelaar, de Waal, Veldhuis Kroeze, Hinchcliffe, Smith, Montomoli, Piccirella, Osterhaus, Knight, Oxford, Lapini, Cox, Lambkin-Williams.
PLoS One. 2014 May 21;9(5):e93761.

Efficacy of live attenuated vaccines against 2009 pandemic H1N1 influenza in ferrets.

Stittelaar KJ, Veldhuis Kroeze EJ, Rudenko L, Dhere R, Thirapakpoomanunt S, Kieny MP, Osterhaus AD.

  Vaccine. 2011 Mar 3;29(11):2120-6. 

Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets.

Baras B, de Waal L, Stittelaar KJ, Jacob V, Giannini S, Kroeze EJ, van den Brand JM, van Amerongen G, Simon JH, Hanon E, Mossman SP, Osterhaus AD.

  Vaccine. 2011 Mar 3;29(11):2092-9. 

Longevity of the protective immune response induced after vaccination with one or two doses of AS03A-adjuvanted split H5N1 vaccine in ferrets.

Baras B, Stittelaar KJ, Kuiken T, Jacob V, Bernhard R, Giannini S, de Waal L, van Amerongen G, Simon JH, Osterhaus AD, Hanon E, Mossman SP.

  J Virol. 2010 Aug;84(16):7943-52.

A single immunization with CoVaccine HT-adjuvanted H5N1 influenza virus vaccine induces protective cellular and humoral immune responses in ferrets.

Bodewes R, Kreijtz JH, van Amerongen G, Geelhoed-Mieras MM, Verburgh RJ, Heldens JG, Bedwell J, van den Brand JM, Kuiken T, van Baalen CA, Fouchier RA, Osterhaus AD, Rimmelzwaan GF.

  Vaccine. 2010 Nov 29;28(51):8125-31.

Feasibility of single-shot H5N1 influenza vaccine in ferrets, macaques and rabbits.

Heldens JG, Glansbeek HL, Hilgers LA, Haenen B, Stittelaar KJ, Osterhaus AD, van den Bosch JF.

  Sci Transl Med. 2009 Dec 23;1(12):12re1.

Seasonal influenza vaccine provides priming for A/H1N1 immunization.

Del Giudice G, Stittelaar KJ, van Amerongen G, Simon J, Osterhaus AD, Stöhr K, Rappuoli R.

  PLoS One. 2008 Jan 2;3(1):e1401.

Cross-protection against lethal H5N1 challenge in ferrets with an adjuvanted pandemic influenza vaccine.

Baras B, Stittelaar KJ, Simon JH, Thoolen RJ, Mossman SP, Pistoor FH, van Amerongen G, Wettendorff MA, Hanon E, Osterhaus AD.