Accelerating COVID-19 research through state-of-the-art preclinical, clinical diagnostics and global supply chain services
Molecular Assays
Real Time qPCR |
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| Isolation: | MagNA Pure 96 (Roche) |
| RT-PCR: | ABI 7500 PCR machines (Thermo Fisher) |
| Results & QC |
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N-gene_1 assay |
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| Target: | N gene |
| Specificity: | SARS-CoV-2 |
| Established by: | CDC (USA) |
| LLOD95: | 2.27 log10 cp/ml |
| Validated range*: | 2.08-9.08 log10 cp/ml |
| WHO recommended | |
* Upper limit not formally established; assay met all criteria at highest concentration available for testing.
E_Sarbeco assay |
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| Target: | E gene |
| Specificity: | betacoronaviruses from SARS-clade (only SARS-CoV-2 occurs in humans) |
| Established by: | Drosten lab (Charité Hospital Berlin, Germany) |
| LLOD95: | 2.33 log10 cp/ml |
| Validated range*: | 1.91-8.91 log10 cp/ml |
| WHO recommended | |
* Upper limit not formally established; assay met all criteria at highest concentration available for testing.
PolHel assay |
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| Target: | Boundary of polymerase and helicase genes |
| Specificity: | SARS-CoV-2 |
| Established by: | Chan JFW et al, J Clin Microbiol 58:e00310-20 (2020) (Hong Kong) |
| LLOD95: | 1.84 log10 cp/ml |
| Validated range*: | 2.08-9.08 log10 cp/ml |
* Upper limit not formally established; assay met all criteria at highest concentration available for testing.
NextGen SequencingTo learn more about our NextGen sequencing capabilities, please visit our sister company’s website DDL Diagnostic Laboratory here. |
If you require any additional information or if you would like to discuss specific queries regarding your upcoming project, please feel free to contact our dedicated team at: info@viroclinics.com
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